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. 2007 Jan;9(1):23–35. doi: 10.1593/neo.06658

Figure 2.

Figure 2

SPARC antagonizes LPA-induced ovarian cancer cell chemotaxis and invasion. LPA (10–50 µM in SFM, added to the bottom chamber of transwell inserts) stimulated the migration of SKOV3 (A) and OVCAR3 (C) cells in a concentration-dependent manner. SPARC (10 µg/ml) added with SKOV3 and OVCAR3 cells to the top chamber of the inserts inhibited LPA-induced migration. Results are expressed as the mean ± SEM of the fold change in migrated cells under experimental conditions, compared to controls attracted by a complete growth medium placed in the bottom chamber (assigned a value of 1). LPA (10–50 µM in SFM) added to SKOV3 (B) and OVCAR3 (D) cells in the top chamber stimulated the invasion of FN-coated inserts and the migration of both cell lines toward a complete growth medium in the bottom chamber. The effect of LPA on FN invasion was significantly inhibited by SPARC. Results are expressed as the mean ± SEM of the fold change in invading cells under experimental conditions, compared to control cells exposed to 0.4% BSA in SFM (assigned a value of 1). Represented are the results of an experiment performed in triplicate and repeated thrice with similar results. *P < .05, compared to unstimulated control. **P < .05, compared to control or LPA stimulation.