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. 2006 Dec 11;51(3):946–957. doi: 10.1128/AAC.01214-06

FIG. 2.

FIG. 2.

(A) Southern blot analysis of XhoI-digested genomic DNA isolated from wild-type Streptomyces sp. strain UC 11065 (WT) and UC11065(dox8::pDOX8hx) using a 995-bp internal dox8 fragment as a probe. The sizes of the predicted and observed hybridizing fragments present in the wild type and in mutant dox8::pDOX8hx are shown. X, XhoI; Ap, apramycin resistance gene. (B) Southern blot analysis of XhoI-digested genomic DNA isolated from wild-type Streptomyces sp. strain UC 11065 (WT) and UC 11065(dox17::pDOX17hx) using a 1,211-bp internal dox17 fragment as a probe. The sizes of the predicted and observed hybridizing fragments present in the wild type and in mutant dox17::pDOX17hx are shown. X, XhoI; Ap, apramycin resistance gene.