TABLE 1.
Bacterial strains and plasmids used in this work
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Bacterial strains | ||
| Actinosporangium vitaminophilum (Streptomyces vitaminophilus) (ATCC 31673) | Producer of pyrrolomycins A to D, wild type | ATCC |
| Streptomyces sp. strain UC 11065 | Producer of dioxapyrrolomycin and pyrrolomycins B to D, wild type | Upjohn Laboratories |
| Streptomyces sp. strain UC 11065(dox8::pDOX8hx) | Streptomyces sp. strain UC 11065 with single-crossover disruption of dox8 | This work |
| Streptomyces sp. strain UC 11065(dox17::pDOX17hx) | Streptomyces sp. strain UC 11065 with single-crossover disruption of dox17 | This work |
| E. coli DH10B | General cloning host | Invitrogen |
| E. coli 12567 | dam-13::Tn9 dcm-6 hsdM | 28 |
| Plasmids | ||
| pBluescript II SK(+) | E. coli cloning vector; Ampr | Stratagene |
| pSP72 | E. coli cloning vector; Ampr | Promega |
| pGEM-T Easy | PCR cloning vector; Ampr | Promega |
| pOJ446 | E. coli-Streptomyces shuttle cosmid for conjugal transfer; Apr | 1 |
| pKC1139 | E. coli-Streptomyces shuttle plasmid for conjugal transfer with temperature-sensitive Streptomyces replicon; Apr | 1 |
| pGEM-T Easy 15001 | 1.5-kb PCR fragment spanning the C terminus of pyr16 and the N terminus of pyr17 in pGEM-T Easy | This work |
| pDOX8hx | 995-bp PCR internal fragment of dox8 in pKC1139 | This work |
| pDOX17hx | 1,211-bp PCR internal fragment of dox17 in pKC1139 | This work |
| pCAV2 | pOJ446 cosmid clone of A. vitaminophilum hybridizing to the pGEM-T Easy 15001 pyr16-pyr17 insert | This work |
| pCAV5 | pOJ446 cosmid clone of A. vitaminophilum hybridizing to the pGEM-T Easy 15001 pyr16-pyr17 insert | This work |
| pCAV2PX3 | pOJ446 cosmid clone of A. vitaminophilum overlapping CAV2 | This work |
| pCAV3PX1 | pOJ446 cosmid clone of A. vitaminophilum overlapping CAV5 | This work |
| pCU13 | pOJ446 cosmid clone of Streptomyces sp. strain UC 11065 hybridizing to the pGEM-T Easy 15001 pyr16-pyr17 insert | This work |