TABLE 2.
Organization and analysis of pyrrolomycin biosynthetic gene cluster from A. vitaminophilum
| Gene | Size (aa) | Protein homolog(s) (GenBank accession number), similarity/identity (%)a | Possible function |
|---|---|---|---|
| orf1 | 280 | FdhD (Q82M23), 82/72 | Formate dehydrogenase |
| pyr2 | 536 | Nfa35380 (BAD58386), 49/37 | Flavin adenine dinucleotide-dependent monooxygenase |
| pyr3 | 206 | Nfa14550 (BAD56300), 51/37 | TetR family regulator |
| pyr4 | 645 | KorA (NP_826054), 90/82 | 2-Oxoglutarate ferredoxin oxidoreductase, α-subunit |
| pyr5 | 355 | KorB (BAC72588), 84/75 | 2-Oxoglutarate ferredoxin oxidoreductase, β-subunit |
| pyr6 | 479 | GntQ (AAR98563), 71/50 | Membrane antiporter |
| pyr7 | 379 | PltE (AAD24879), 70/56 | Pyrrole synthesis |
| pyr8 | 470 | STIAU_0873 (EAU66332), 64/51; PltF (AAD24881), 63/51 | adenylation of l-proline |
| pyr9 | 187 | Amb2577 (BAE51381), 63/44 | Flavin reductase |
| pyr10 | 99 | Bpro_5327 (ABE47183), 60/42 | Acyl carrier protein |
| pyr11 | 557 | HalA (AAQ04684), 53/37; PltD (AAD24878), 52/34 | Halogenase(?); FADH2 motifs absent |
| pyr12 | 289 | SAV3633 (NP_824810), 62/46 | Unknown |
| pyr13 | 260 | TTC1745 (AAS82087), 55/37 | Oxidoreductase |
| pyr14 | 172 | Mlr2550 (BAB49655), 63/46 | Unknown |
| pyr15 | 313 | PA3330 (AAG06718), 56/42 | Oxidoreductase |
| pyr16 | 584 | HalA (AAQ04684), 80/69 | Halogenase |
| pyr17 | 552 | HalB (AAQ04685), 75/59 | Halogenase |
| pyr18 | 694 | NasA (CAC19469), 76/66 | Assimilatory nitrate reductase, catalytic subunit |
| pyr19 | 473 | NasC (AAK77360), 62/49 | Assimilatory nitrate reductase, electron transfer subunit |
| pyr20 | 405 | NocL (AAT09797), 62/44 | Cytochrome P450 |
| pyr21 | 104 | TcmH (AAA67512), 71/51 | Monooxygenase |
| pyr22 | 232 | MAP0125c (NP_959059), 49/36 | Methyl transferase |
| pyr23 | 355 | Tmn22 (BAE93743), 54/37 | Membrane protein |
| pyr24 | 2,051 | PltC (AAC38076) 58/42 | Polyketide synthase |
| pyr25 | 2167 | PltB (AAC38075), 57/43 | Polyketide synthase |
| pyr26 | 302 | LmnN (AAN85527), 58/43; PltG (AAD24880), 49/31 | Thioesterase |
| pyr27 | 207 | SCO3346 (CAB42672), 60/41 | TetR family regulator |
| pyr28 | 90 | PltL (AAQ90170), 69/41 | Proline carrier protein |
| pyr29 | 447 | ChlB4 (AAZ77674), 82/69; PltA (AAD24884), 78/65 | Halogenase |
| pyr30 | 297 | Orf56 (BAC76514), 61/48 | PPTase |
| pyr31 | 594 | Orf57 (BAC76515), 54/44 | Acyl CoA dehydrogenase |
| orf32 | 480 | SCO1715 (CAB50930), 83/75 | Homogentisate 1,2-dioxygenase |
| orf33 | 250 | SCO1716 (CAB50931), 81/70 | GntR family regulatory protein |
| orf34 | 383 | MvanDRAFT_5628 | Acyl CoA dehydrogenase |
| orf35 | 387 | Bcep18194_C7158 (AAB06202), 69/56 | l-Carnitine dehydratase |
a
plt genes are from the pyoluteorin gene cluster of P. fluorescens.