TABLE 3.
Organization and analysis of the partial pyrrolomycin biosynthetic gene cluster from Streptomyces sp. strain UC 11065
| Gene | Size (aa) | Protein homolog (accession number), similarity/identity (%) | Possible function (% identity to A. vitaminophilum protein homolog) |
|---|---|---|---|
| dox5 | 374 | SCO4594 (CAC08295), 86/80 | 2-Oxoglutarate ferredoxin oxidoreductase, β-subunit (77) |
| dox6 | 479 | GntQ (AAR98563), 68/49 | Membrane antiporter (76) |
| dox7 | 379 | PltE (AAD24879), 69/55 | Pyrrole synthesis (87) |
| dox8 | 489 | CloN4 (AAN65233), 64/52 | Adenylation of l-proline (86) |
| dox9 | 187 | AvinDRAFT_6224 (EAM07574), 60/43 | Flavin reductase (81) |
| dox10 | 98 | Ava_4741 (ABA24338), 56/36 | Acyl carrier protein (56) |
| dox16 | 583 | HalA (AAQ04684), 81/69 | Halogenase (87) |
| dox17 | 552 | HalB (AAQ04685), 75/59 | Halogenase (92) |
| dox18 | 735 | NasA (CAC19469), 76/66 | Assimilatory nitrate reductase, catalytic subunit (79) |
| dox19 | 477 | NasC (AAK77360), 59/49 | Assimilatory nitrate reductase, electron transfer subunit (68) |
| dox19A | 327 | med-Orf21 (BAC79034), 73/65 | Kinase (absent from A. vitaminophilum) |
| dox19B | 148 | MflvDRAFT_0738 (EAS08754), 55/41 | Methyltransferase (absent from A. vitaminophilum) |
| dox20 | 405 | NocL (AAT09797), 59/44 | Cytochrome P450 (65) |
| dox21 | 103 | Nwi_2139 (ABA05393), 62/47 | Monooxygenase (76) |
| dox22 | 226 | MAP_2796c (AAS05113), 60/46 | Methyl transferase (59) |
| dox23 | 353 | Tmn22 (BAE93743), 50/35 | Membrane protein (45) |
| dox24 | 2,067 | MxaE (AAK57189), 48/34 | Polyketide synthase (82) |