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Primer sequences utilized in the quantitative PCR analysis
| Gene | Primera | Sequence (5′→3′) | Accession no. | Primer concn (nM) |
|---|---|---|---|---|
| TNF-α gene | FW | GGACCTCTCTCTAATCAGCCCTC | NM_000594 | 25 |
| RV | TCGAGAAGATGATCTGACTGCC | 25 | ||
| IL-6 gene | FW | CGGTACATCCTCGACGGC | NM_000600 | 25 |
| RV | CTTGTTACATGTCTCCTTTCTCAGG | 450 | ||
| GAPDH gene | FW | GTGGAGTCCACTGGCGTCTT | J04038 | 25 |
| RV | GCAAATGAGCCCAGCCTTC | 150 |
FW, forward; RW, reverse.
