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LacZ expression and inhibition with antisense peptide-PNA conjugate in K. pneumoniae. The values indicate relative enzyme activities in K. pneumoniae cultures growing in MHB. For antisense inhibition, the lacZ gene in K. pneumoniae was induced with 50 μM IPTG and assayed at A420 with the substrate ONPG. Anti-lacZ, antisense peptide-PNA targeted at lacZ gene; Mis-lacZ, mismatched peptide-PNA targeted at lacZ gene.
