FIG. 4.
Dose-dependent modulating activity of flavonoid dimers 9c and 9d on the pentamidine resistance of LePentR50. LePentR50 promastigotes were seeded at 105 cells/well in 100 μl and then incubated at 27°C for 72 h with either flavonoid dimers only (9c only [A] or 9d only [B]) or together with pentamidine (9c with 60 μg of pentamidine/ml [A] or 9d with 60 μg of pentamidine/ml [B]). The percentage of survivors was measured by using an MTS assay. Each concentration of 9c or 9d was tested in triplicate, and analyses were repeated three times.