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Effect of 9d on pentamidine accumulation of LePentR50. A 1-ml portion of 4-day-old LePentR50 promastigotes (2 × 108 cells/ml) was incubated at 27°C for 3 h with 0.84 mM pentamidine in the presence of various concentrations of 9d (0, 15, 30, and 60 μM). After incubation, cells were washed with PBS, and the pentamidine concentration was determined by HPLC. Each concentration of 9d was tested in triplicate, and analyses were repeated twice.
