TABLE 1.
Oligonucleotides used for gene sequencing and evaluation of gene expression
| Gene name (gene product) | GenBank accession no. | Primer | Nucleotide sequence (5′-3′) | Nucleotide coordinatesa |
|---|---|---|---|---|
| CgERG4 (C-24[28] sterol reductase) | NC005967 | ERG4-1F | CAACACAATAATCGGTGGGGT | 44666-44686 |
| ERG4-1R | CAAGGATGTTACAATAATGCA | 44082-44102 | ||
| ERG4-2F | CTCCAGTGATAAACATAAACG | 44228-44248 | ||
| ERG4-2R | AAACACCTGGCAGAGTGTAA | 43654-43673 | ||
| ERG4-3Fb | TCATCGGCAAACTTTACCA | 43794-43812 | ||
| ERG4-3Rb | AGCCATATGTTTCATATTGCT | 43333-43353 | ||
| ERG4-4F | TTGACTTGAAGATGTTCTTCG | 43380-43400 | ||
| ERG4-4R | TGATCCATTGGAAGTGACCA | 42895-42914 | ||
| ERG4-5F | TGTCTGGCGATAAGACTGTCA | 42945-42965 | ||
| ERG4-5R | CCCATGAACCGTTTTTCCTT | 42414-42433 | ||
| CgERG5 (C-22 sterol desaturase) | NC006036 | ERG5-1F | TTGACTACCGTATCGGGGATT | 765352-765372 |
| ERG5-1R | TGGCATTCATGCTCATATGC | 765995-765814 | ||
| ERG5-2Fb | TGCAATTGGGATACCCGATT | 765699-765718 | ||
| ERG5-2Rb | CCATCAATTCTTCCAAAGGAG | 766301-766321 | ||
| ERG5-3F | AAAGCCCACACCGACTATAGA | 766231-766251 | ||
| ERG5-3R | GCATCTTGAGAAGCGAACAA | 766762-766781 | ||
| ERG5-4F | AACCGTACCGACGATGACTCT | 766684-766704 | ||
| ERG5-4R | ACCGATCAAAGCAGTCATGGT | 767215-767235 | ||
| ERG5-5F | TCCACACGTTTGTCTAGGTCA | 767178-767198 | ||
| ERG5-5R | CTGGGAAAGATTTGCAATAGG | 767510-767530 | ||
| CgERG6 (Δ[24] sterol C-methyltransferase) | NC006031 | ERG6-1F | CGGCATTTGGATTTTCTCGT | 444995-445014 |
| ERG6-1R | TCGGGAGAATTTCAATTCC | 445490-445508 | ||
| ERG6-2F | CAGTTTATTGTGCTCTTGACG | 445443-445463 | ||
| ERG6-2R | TGAATCTGGCGATGGTACG | 445932-445949 | ||
| ERG6-3Fb | GCATACATGGCCGGTATCAA | 445862-445881 | ||
| ERG6-3Rb | ACTTCCATTCACCGGTCAAT | 446395-446414 | ||
| ERG6-4F | TTTGAAGAACGTCGGTTCG | 446317-446336 | ||
| ERG6-4R | CATGTGGAATGAATTCAAGTG | 446821-446841 | ||
| CgERG11 (lanosterol 14-α demethylase) | L40389 | ERG11-1F | CTACAATCGAGTGAGCTTG | 17-35 |
| ERG11-1R | GTAGAACACAAGTGGTGG | 729-746 | ||
| ERG11-2F | GGTCGTTGAACTATTGGAG | 584-602 | ||
| ERG11-2R | GGACCCAAGTAGACAGTC | 863-880 | ||
| ERG11-3Fb | CCATCACATGGCAATTGC | 688-705 | ||
| ERG11-3Rb | GGTCATCTTAGTACCATCC | 1445-1463 | ||
| ERG11-4F | CGTGAGAAGAACGATATCC | 1380-1398 | ||
| ERG11-4R | CACCTTCAGTTGGGTAAC | 2047-2064 | ||
| ERG11-5F | CGCTTACTGTCAATTGGG | 1991-2008 | ||
| ERG11-5R | GTCATATGCTTGCACTGC | 2397-2414 | ||
| CgERG9 (squalene synthase) | AB009978 | ERG9 Fb | TGATTCTGAGGGCTTTGGA | 725-743 |
| ERG9 Rb | TACAATCGACACCCTTTTGG | 1293-1312 | ||
| CgERG1 (squalene epoxydase) | AF006033 | ERG1 Fb | CGTTGCTTTTGTTCATGGTAG | 1-21 |
| ERG1 Rb | ATACCACCACCAGTTAGAGGG | 589-609 | ||
| CgERG2 (C-8 sterol isomerase) | NC006035 | ERG2 Fb | TGTACTTGCCAAACACAACG | 1142387-1142406 |
| ERG2 Rb | ACAAATCCAAAGTGGAGGAGA | 1142840-1142860 | ||
| CgERG3 (C-5 sterol desaturase) | L40390 | ERG3 Fb | AAGATTGCGCCTGTTGAGTT | 615-634 |
| ERG3 Rb | TACCACAGTCGGTGAAGAAGA | 1129-1149 | ||
| CgCDR1 (ABC transporter) | AF109723 | CDR1 Fb | GAAGTCTATGGAAGGTGC | 1084-1101 |
| CDR1 Rb | GTCTAGCGTAAGTCTCTC | 1383-1400 | ||
| CgCDR2 (ABC transporter) | AF251023 | CDR2 Fb | GTTGAGTACTGGCACAAC | 362-379 |
| CDR2 Rb | GATGGCAAAGAACATGGC | 695-712 | ||
| CgSTE12 (MAP-activated transcription factor) | AJ515385 | STE12 Fb | AAGCTGTAACACATCTCATGC | 855-875 |
| STE12 Rb | AACAGGAGTCATATGCGAAG | 1472-1491 | ||
| CgACT (β actin) | AF069746 | ACT Fb | TATTGACAACGGTTCCGG | 949-966 |
| ACT Rb | TAGAAAGTGTGATGCCAG | 1177-1194 |
a
Nucleotide coordinates refer to the corresponding gene sequence in the GenBank database.
b
Primers used for evaluation of gene expression.