Figure 5.

In situ autoradiograms (A and B) and fluorescence micrographs (C–H) of E13 explants treated either with 1.5 μM nonsense-ODN (A, C, E, and G) or TIN-ag antisense-ODN (B, D, F, and H). The GLEPP1 mRNA is localized to glomeruli, and no decrease in their population is observed after the antisense treatment (B vs. A, arrows). There were no changes in anti-HS-PG antibody reactivity (D vs. C), and WGA staining (F vs. E) is noted between the two groups, although a tubular (t) loss is observed in the antisense group. The TIN-ag antibody reactivity is seen in the tubular basement membranes (G), and a mild reduction is observed in the remaining tubules after antisense treatment (H). u, ureteric bud branches. (A and B, ×40; C–H, ×80.)