Table 1.
Summary of cell-cycle kinetics of MAPKK-mutant expressing cell lines
| Cell line | S phase, hr | G2/M phase, hr | G1 phase, hr |
|---|---|---|---|
| Mock-1 | 6.2 ± 0.2 | 3.0 ± 0.5 | 6.0 |
| Mock 2 | 5.9 ± 0.1 | 3.2 ± 0.1 | 6.5 ± 0.5 |
| Mock-9 | 5.9 ± 0.1 | 3.5 ± 0.1 | 5.9 ± 0.3 |
| S222A-13* | 6.0 ± 0.2 | 5.9 ± 0.1 | ND |
| S222A-21 | 6.5 ± 0.3 | 6.2 ± 0.4 | ND |
| K97A-2 | 7.0 ± 0.0 | 6.7 ± 0.2 | ND |
| S222E-1 | 5.8 ± 0.2 | 3.9 ± 0.2 | 4.6 ± 0.3 |
| S22E-8 | 6.0† | 3.5 | 4.5 |
| WT-31 | 6.0 ± 0.0 | 4.3 ± 0.3 | 7.0 |
| WT-32 | 6.0 ± 0.0 | 3.7 ± 0.3 | 7.0 |
*
Each clone is labeled with the letters of the mutation, followed by the cell clone number assigned in the original isolation (e.g., S222A-13).
ND, not determined.
†
For some clones, the timine of cell-cycle progression was measured fewer than three times and standard error could not be calculated.