Table I.
Segregation of Mi-9 phenotype and linked markers in F2 population of S. arcanum LA2157 × LA392
Plants were genotyped using PCR and RFLP markers. Genotype designation: (1) homozygous resistant locus, (2) homozygous susceptible, (3) heterozygous, 1/3 resistant allele is dominant, and 2/3 susceptible allele is dominant. Plants were also evaluated for nematode resistance (R) or susceptibility (S) to root-knot nematodes strain VW4 at 32°C.
| Plant | Attributes | No. of Plants | Phenotype | Markers
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| CT119 | Cf-2 | Rex-1 | C264.1 | C32.1 | C8B | Aps-1 | ||||
| LA2157 | Parent | – | R | 1 | 1 | 1 | 1/3 | 1 | 1/3 | 1 |
| LA392 | Parent | – | S | 2 | 2 | 2 | 2 | 2/3 | 2 | 2 |
| F2 class 1 | Parental type | 124 | R | 1 | 1 | 1 | nda | nd | 1/3 | 1 |
| F2 class 2 | Parental type | 145 | S | 2 | 2 | 2 | nd | nd | 2 | 2 |
| F2 class 3 | Heterozygote | 264 | R | 3 | 3 | 3 | nd | nd | 1/3 | 3 |
| F2 class 4 | Recombinant | 6 | R | 3 | 3 | 3 | 1/3 | 2/3 | 3b | 1 |
| F2 class 5 | Recombinant | 8 | R | 3 | 3 | 3 | 1/3 | 2/3 | 3b | 2 |
| F2 class 6 | Recombinant | 5 | S | 2 | 2 | 2 | 2 | 2/3 | 2 | 3 |
| F2 class 7 | Recombinant | 11 | R | 1 | 1 | 1 | 1/3 | 1/1 | 1b | 3 |
| F2 class 8 | Recombinant | 5 | R | 3 | 3 | 3 | 1/3 | 2/3 | 1b | 1 |
| F2 class 9 | Recombinant | 1 | R | 2 | 2 | 2 | 2 | 3b | 3b | 3 |
| F2 class 10 | Recombinant | 1 | R | 1 | 1 | 1 | 3b | 3b | 3b | 3 |
| F2 class 11 | Recombinant | 8 | R | 2 | 2 | 3 | 1/3 | 2/3 | 3b | 3 |
| F2 class 12 | Recombinant | 2 | S | 3 | 3 | 2 | 2 | 2/3 | 2 | 2 |
| F2 class 13 | Recombinant | 1c | R | 1 | 3 | 3 | nd | nd | 1/3 | 3 |
| F2 class 14 | Recombinant | 6 | R | 2 | 3 | 3 | nd | nd | 1/3 | 3 |
a
nd, Not determined.
b
Allele designation is based on F3 progeny segregation.
c
Plant died before setting fruits.