Table I.
Changes in expression of genes involved in ABA biosynthesis or ABA signal transduction in Arabidopsis accession Col-0 treated with air or ethylene (5 μ L L−1) for 3 h using Affymetrix GeneChips
Values (in arbitrary units) represent means with standard errors of three independent biological replicates. Phospolipases are named according to Qin and Wang (2002). Small GTPases are named according to Yang (2002). Symbols for P values: b.t., Below threshold; n.s, not significant; *, 0.05 > P > 0.01; **, P < 0.01.
| Locus | Gene Code | Expression Air | Expression Ethylene | P | Percent Change |
|---|---|---|---|---|---|
| ABA biosynthesis | |||||
| ABA1 (ZEP) | At5g67030 | 1,512 ± 56 | 1,380 ± 94 | n.s. | |
| NCED1 | At3g63520 | 1,321 ± 77 | 1,283 ± 69 | n.s. | |
| NCED2 | At4g18350 | b.t. | b.t. | ||
| NCED3 | At3g14440 | b.t. | b.t. | ||
| NCED4 | At4g19170 | 1,055 ± 114 | 703 ± 65 | n.s. | |
| NCED5 | At1g30100 | b.t. | b.t. | ||
| NCED6 | At3g24220 | b.t. | b.t. | ||
| NCED9 | At1g78390 | b.t. | b.t. | ||
| ABA2 | At1g52340 | 328 ± 11 | 297 ± 9 | n.s. | |
| ABA3 | At1g16540 | b.t. | b.t. | ||
| AAO3 | At2g27150 | 100 ± 3 | 107 ± 2 | n.s. | |
| ABA catabolism | |||||
| CYP707A1 | At4g19230 | 80 ± 8 | 69 ± 2 | n.s. | |
| CYP707A2 | At2g29090 | 112 ± 5 | 111 ± 3 | n.s. | |
| CYP707A3 | At5g45340 | 115 ± 7 | 121 ± 3 | n.s. | |
| CYP707A4 | At3g19270 | b.t. | b.t. | ||
| Signal transduction | |||||
| ABI1 | At4g26080 | 355 ± 31 | 919 ± 18 | ** | 159 |
| ABI2 | At5g57050 | 98 ± 4 | 105 ± 4 | n.s. | |
| ABI3 | At3g24650 | 52 ± 2 | 50 ± 1 | n.s. | |
| ABI4 | At2g40220 | 78 ± 3 | 77 ± 7 | n.s. | |
| ABI5 | At2g36270 | 64 ± 3 | 67 ± 1 | n.s. | |
| ERA1 | At5g40280 | 174 ± 1 | 181 ± 11 | n.s. | |
| EIN2 (ERA3) | At5g03280 | 608 ± 18 | 563 ± 28 | n.s. | |
| FUS3 | At3g26790 | b.t. | b.t. | ||
| LEC1 | At1g21970 | 53 ± 1 | 50 ± 2 | n.s. | |
| FRY1 | At5g63980 | 1,408 ± 66 | 1,214 ± 30 | n.s. | |
| HYL1 | At1g09700 | b.t. | b.t. | ||
| ABH1 | At2g13540 | 308 ± 5 | 319 ± 10 | n.s. | |
| IP5PI | At1g34120 | 83 ± 1 | 92 ± 1 | n.s. | |
| IP5PII | At4g18010 | 97 ± 2 | 98 ± 5 | n.s. | |
| P2C-HA | At1g07430 | b.t. | b.t. | ||
| PP2C | At3g11410 | 385 ± 25 | 311 ± 7 | n.s. | |
| CDPK1 | At1g18890 | 147 ± 15 | 152 ± 7 | n.s. | |
| CDPK1a | At1g74740 | 55 ± 4 | 52 ± 4 | n.s. | |
| RAB18 | At5g66400 | 80 ± 6 | 69 ± 3 | n.s. | |
| Phospholipases | |||||
| PLC1 | At5g58670 | 1,014 ± 23 | 635 ± 19 | ** | −37 |
| PLC2 | At3g08510 | 2,764 ± 94 | 2,548 ± 155 | n.s. | |
| PLD a1 | At3g15730 | 1,721 ± 135 | 1,523 ± 44 | n.s. | |
| PLD a2 | At1g52570 | 160 ± 5 | 158 ± 6 | n.s. | |
| PLD a3 | At5g25370 | 144 ± 13 | 135 ± 11 | n.s. | |
| PLD a4 | At1g55180 | 110 ± 11 | 102 ± 8 | n.s. | |
| PLD b1 | At2g42010 | 124 ± 11 | 131 ± 7 | n.s. | |
| PLD b2 | At4g00240 | b.t. | b.t. | ||
| Small GTPases | |||||
| ROP2 | At1g20090 | 523 ± 37 | 382 ± 46 | n.s. | |
| ROP5 | At4g35950 | 455 ± 20 | 409 ± 29 | n.s. | |
| ROP6 | At4g35020 | 287 ± 19 | 264 ± 14 | n.s. | |
| ROP9 | At4g28950 | 90 ± 4 | 79 ± 2 | n.s. | |
| ROP10 | At3g48040 | 191 ± 12 | 191 ± 4 | n.s. |