Figure 4.

Complementation of Arabidopsis sos1-1 mutant by rice OsSOS1. A, Six-day-old seedlings grown on Murashige and Skoog agar medium were transferred to Murashige and Skoog medium supplemented with 50 mm NaCl and imaged after 14 d of salt treatment. Left, Transgenic sos1-1 mutant seedling expressing AtSOS1 from the cauliflower mosaic virus 35S promoter. Middle, five independent transgenic lines of sos1-1 mutants expressing the rice OsSOS1 gene under the control of the 35S promoter. Right, sos1-1 mutant seedling transformed with empty vector pBI321. B, Quantitation of seedling growth, expressed as fresh weight after 14 d in Murashige and Skoog medium with and without supplemental 65 mm NaCl. Data are the mean and se of fresh-weight values of three to six individual seedlings from each line. Dashed bars, sos1-1 mutant seedlings transformed with empty vector pBI321; gray bars, sos1-1 mutant seedlings transformed with the rice OsSOS1 gene under the control of the 35S promoter; black bars, sos1-1 mutant seedlings transformed with Arabidopsis SOS1. [See online article for color version of this figure.]