Figure 6.
Functional interactions between Arabidopsis SOS proteins and rice counterparts. Strains YP890 carrying the integration of the PGK1PRO:AtSOS1:CYC1TER cassette (A and C), YP1021 with the analogous integration PMA1PRO:OsSOS1:ADH1TER with the SOS1 cDNA from rice (D), and AXT3K cells transformed with plasmid pSOS1-1 for the expression of AtSOS1 (B) were transformed with plasmids directing the expression of the regulatory proteins SOS2 and SOS3 from Arabidopsis or CIPK24 and CBL4 from rice, as indicated in each case. CIPK24Δ309 bears a C-terminal deletion rendering a constitutive, CBL-independent protein kinase. Yeast cells were grown overnight in selective synthetic dextrose medium. Five microliters of serial decimal dilutions were spotted onto plates of AP medium with 1 mm KCl and 200 mm NaCl. Plates were incubated at 28°C for 3 to 4 d.