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Kinetic parameters for IPMS1 and IPMS2
| Enzyme | Substrate | Km (μm) ± se | Vmax (μmol min−1 g−1) ± se | kcat (s−1) | kcat/Km (m−1 s−1) |
|---|---|---|---|---|---|
| IPMS1 | 2-Oxoisovaleratea | 304 ± 68 | 2.3 × 103 ± 0.2 × 103 | 2.4 | 7.8 × 103 |
| Acetyl-CoAb | 45 ± 10 | 2.1 × 103 ± 0.1 × 103 | 2.2 | 4.7 × 104 | |
| IPMS2 | 2-Oxoisovaleratea | 279 ± 51 | 2.2 × 103 ± 0.1 × 103 | 2.3 | 8.3 × 103 |
| Acetyl-CoAb | 16 ± 4 | 1.8 × 103 ± 0.1 × 103 | 1.9 | 1.2 × 105 |
Measured in the presence of 500 μm acetyl-CoA.
Measured in the presence of 1 mm 2-oxoisovalerate.
