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Kinetic constants for UGT74M1 and various sapogenins substrates
See “Materials and Methods” for details.
| Substrate |  |
 |
 |
|---|---|---|---|
| μm | s−1 | s−1m−1 | |
| Gypsogenic acida | 170 | 1.13 | 6.5 × 103 |
| 16-OH gypsogenic acid | 51 | 1.29 | 2.5 × 104 |
| Gypsogenin | 42 | 0.125 | 3.0 × 103 |
| Quillaic acid | 37 | 0.111 | 3.0 × 103 |
Constants for gypsogenic acid are considered estimates, because solubility limited the maximum concentration used to 100 μm.
