Figure 3.

cul1-6 seedlings display reduced auxin response. A, Four-day-old wild-type (wt) and cul1-6 seedlings were transferred to medium with or without 0.1 μm 2,4-D and grown for an additional 4 d. B, Root growth of wt, cul1-6, axr6-1/+, and axr6-3 seedlings on increasing concentrations of 2,4-D was measured and expressed as percentage of root length on control plates. n = 20; error bars ± se. C, Number of lateral roots produced by 10-d-old wt and cul1-6 seedlings grown on plates with and without 0.1 μm 2,4-D for 5 d. n = 10; error bars ± se. D, BA3-GUS expression induced by 20 μm auxin treatment in wt or cul1-6 seedling roots. E and F, HS-AXR3NT-GUS expression in cotyledons (E) or roots (F) of wt seedlings or cul1-6 seedlings.