Figure 1.

Yeast two-hybrid assays between five barley 14-3-3 isoforms and the 132 identified 14-3-3 interactors. All the 132 unique 14-3-3 interactors identified in the yeast two-hybrid screens were retransformed into yeast carrying one of the barley 14-3-3 isoforms and are numbered from spot 2 to 133. Interaction is confirmed by growing the double transformants on selective media (top; SD minus LWHA) or performing β-galactosidase assay (bottom). As control, empty-BD constructs were used against the 14-3-3 interactors (top lane) and empty-AD against the 14-3-3-BD (spot 1).