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. 2007 Feb;143(2):670–683. doi: 10.1104/pp.106.090159

Figure 3.

Figure 3.

Three ABF transcription factors interact with 14-3-3 proteins. A, Quantitative β-galactosidase activity of yeast cells carrying the 14-3-3 proteins as bait and the ABF transcription factors as prey. Eight amino acid deletions at the C terminus (C8Del) were tested for their interaction with the 14-3-3 proteins. B, Semiquantitative β-galactosidase activity assay of double-transformed yeast cells carrying 14-3-3 proteins as bait and the ABF transcription factors (wild type and C8Del) as prey. C, Qualitative assays of the 14-3-3 interactions with the ABF transcription factors (wild type and C8Del). D, Interaction between recombinant protein of the transcription factors HvABF1, HvABF2, and HvABF3 and recombinant 14-3-3C is shown by performing far-western analysis. Glutathione S-transferase-R18 was taken along as positive control. [See online article for color version of this figure.]