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Elutions of 14-3-3 interactors. 14-3-3 interacting proteins were specifically eluted using 0.5 mm R18 peptide. The mock elution, R18 elution, and the imidazol strip were concentrated, and 10% of the samples were separated on a 12% SDS-PAGE gel. Proteins were stained with Bio-Rad Biosafe colloidal Coomassie.
