Table I.
Identified cDNA clones from the 14-3-3 yeast two-hybrid screen
The clones that showed activation of the reporter genes were selected and DNA was isolated. Subsequent sequences were BLAST searched against the TIGR barley expressed sequence tag (TIGR 9.0) database (http://www.tigr.org/tigr-scripts/tgi/T_index.cgi?species=barley).
| Names of Homologous Proteins | TC No.a | Spot No. |
|---|---|---|
| Carbohydrate metabolism | ||
| Rubisco small subunit (wheat) | 131295 | 3 |
| Neutral invertase (Arabidopsis) | 137088 | 43 |
| Cytosolic 6-phosphogluconate dehydrogenase (rice) | 146849 | 84 |
| Dihydrolipoamide S-acetyltransferase (Arabidopsis) | 148282 | 107 |
| Oxidative stress | ||
| Manganese superoxide dismutase (wheat) | 138696 | 5 |
| GCN5-related N-acetyltransferase (rice) | 139926 | 10 |
| Glutathione S-transferase (rice) | 151451 | 70 |
| Cytosolic glutathione reductase (wheat) | 131783 | 132 |
| Signaling | ||
| BRI1-KD interacting protein 129 (rice) | 132082 | 12 |
| Hv14-3-3B (barley) | 132090 | 15 |
| Putative bZIP transcription factor ABI5 (Arabidopsis) | 144090 | 13 |
| ABA response element binding factor (wheat) | 141259 | 20 |
| ABA-responsive element binding factor (Arabidopsis) | 134245 | 49 |
| Phytoene desaturase (barley) | 139909 | 26 |
| Mybst1 (rice) | 142444 | 21 |
| Serpin (barley) | 151425 | 22 |
| WPK4 protein kinase (wheat) | CA019527* | 28 |
| Vacuolar proton-inorganic pyrophosphatase (barley) | 131805 | 11 |
| Adr11 (auxin down-regulated gene 11; soybean) | 147470 | 27 |
| Putative stearoyl-acyl-carrier protein desaturase (rice) | 139480 | 76 |
| Rf2a (rice) | 152053 | 29 |
| Mybs3 (rice) | 140561 | 111 |
| GA-stimulated transcript 1-like protein (rice) | 148126 | 97 |
| RRJ1 (jasmonic acid responsive; rice) | 140455 | 82 |
| WERBP-1 protein (MYB-like DNA-binding domain; tobacco) | 140267 | 118 |
| Adenine phosphoribosyltransferase (barley) | 139680 | 62 |
| Orf2 (Sh1orf2) | BQ463442* | 32 |
| Helix-loop-helix protein (rice) | 148621 | 39 |
| Xyloglucan endotransglycosylase (barley) | 139640 | 46 |
| Putative NPH3 protein (rice) | 136796 | 48 |
| GT-1 (Arabidopsis) | CB883300* | 60 |
| Katanin (rice) | 140752 | 59 |
| Jasmonate-induced mRNA (barley) | 131671 | 66 |
| Phospholipase DΔ isoform 1b-like protein (rice) | 143006 | 116 |
| PIR (GTPase activating-like protein; Arabidopsis) | 140036 | 38 |
| GTP-binding protein (GTP1; rice) | 146421 | 37 |
| RING-H2 zinc protein | BQ460881* | 67 |
| Putative nonphototropic hypocotyl 3 (NPH3) | BE421015* | 65 |
| PHD finger transcription factor-like (rice) | 135529 | 51 |
| Putative Golgi-localized protein (rice) | BU993045* | 52 |
| PHD finger transcription factor-like | XM476784* | 75 |
| Glycosyl hydrolase family (rice) | 146693 | 36 |
| Mpv17 transgene-like (Arabidopsis) | 140218 | 77 |
| Oswrky47 (rice) | 152887 | 78 |
| Putative lipase class 3 family (rice) | 152325 | 24 |
| Zeta1-COP (rice) | 134145 | 81 |
| Similar to adenylate cyclase (trypanosoma) | 140534 | 71 |
| Similar to putative receptor protein kinase PERK1 (rice) | BE519502* | 72 |
| Putative AP2/EREBP domain transcription factor (Arabidopsis) | 145625 | 44 |
| Probable auxin transport protein (PIN1; Arabidopsis) | BG309386* | 93 |
| Putative guanylate kinase (Arabidopsis) | 142364 | 94 |
| Guanine nucleotide-binding protein β-subunit-like protein (rice) | 130838 | 105 |
| Nph3 (barley) | BF617760* | 127 |
| 12-Oxophytodienoate reductase 3 (rice) | 140942 | 133 |
| Putative P23 co-chaperone (rice) | 139577 | 56 |
| Protein synthesis/DNA- and RNA-binding proteins | ||
| 40S ribosomal protein S30-like (rice) | 131072 | 14 |
| Putative 60S ribosomal protein L31 (rice) | 130806 | 55 |
| Eukaryotic translation initiation Factor 6 (Arabidopsis) | 139393 | 89 |
| 9S ribosomal protein | BU981956* | 99 |
| Nonribosomal peptide synthetase modules and related proteins-like (rice) | 141489 | 103 |
| Elongation factor 1α (wheat) | 146580 | 123 |
| Rnase S-like protein (barley) | 140096 | 23 |
| Homolog to histone H3.2 protein (human) | 131253 | 61 |
| RNA-binding protein | BI960230* | 115 |
| Chloroplast proteins | ||
| Inositol phosphatase-like protein (rice) | 146317 | 7 |
| PSI reaction center subunit XI, chloroplast precursor (barley) | 146765 | 19 |
| Chlorophyll a/b-binding protein (barley) | 138667 | 33 |
| PRF light-harvesting (barley) | 139104 | 31 |
| Thylakoid lumenal 35.8-kD protein, chloroplast precursor (Arabidopsis) | 141769 | 58 |
| Chlorophyll a/b-binding protein of LHCII type III (barley) | 146726 | 68 |
| Chlorophyll a/b-binding protein (Hvlhca1; barley) | 138676 | 108 |
| Chlorophyll a/b-binding protein (Lhcb1-2; potato) | 146500 | 219 |
| Putative PSII core complex proteins Psby, chloroplast (rice) | 130812 | 86 |
| Chlorophyll a/b-binding protein (wheat) | 146521 | 25 |
| Tic62 protein | BF267751* | 83 |
| Putative Crp1 protein (rice) | 148944 | 119 |
| Signal recognition particle 54-kD subunit (barley) | 132186 | 126 |
| Ferredoxin, chloroplast precursors (wheat) | 146610 | 17 |
| Ferredoxin-thioredoxin reductase subunit A (maize) | 134446 | 63 |
| Stress-related proteins | ||
| Putative aldehyde dehydrogenase (rice) | BI953625* | 50 |
| Fatty acid hydroperoxide lyase (barley) | 141847 | 100 |
| Chitin-binding lectin 1 precursor (Chlamydomonas) | 137023 | 95 |
| Carboxymethylenebutenolidase-like protein (Arabidopsis) | 139656 | 64 |
| Cell division and apoptosis | ||
| Pelota (rice) | 134531 | 104 |
| Cytokinesis 2 protein (yeast) | 149830 | 47 |
| P14arf protein | CA018141* | 114 |
| Annexin (rice) | 134070 | 131 |
| Proteasome-related proteins | ||
| 26S proteasome regulatory particle non-ATPase subunit-8 (rice) | 147090 | 85 |
| F-box family protein-like (rice) | 145485 | 45 |
| Ubiquitin-conjugating enzyme E2-17 kD (wheat) | 139190 | 110 |
| 26S protease regulatory subunit 7 (rice) | 131810 | 2 |
| Proteolysis | ||
| Degp2 protease (Arabidopsis) | 149647 | 124 |
| ATP-dependent Clp protease (rice) | 139897 | 34 |
| ATPase | ||
| P-type H+-ATPase (wheat) | 139045 | 54 |
| ATPase-like protein (sorghum) | 150845 | 18 |
| Cell wall-associated proteins | ||
| Putative protein (rice) | 132497 | 16 |
| Extensin (Volvox carteri) | BG365257* | 79 |
| Pherophorin-Dz1 protein | BI948976* | 74 |
| Putative β-1,3-glucanase (rice) | 148136 | 106 |
| 3-Glucanase | BF625748* | 102 |
| β-1,3-Glucanase (rice) | 152166 | 69 |
| Putative extensin (rice) | 135984 | 91 |
| Vegetative cell wall protein Gp1 precursor | AV834747* | 109 |
| Extensin-like protein precursor (maize) | 141562 | 117 |
| Hydroxy-Pro-rich glycoprotein DZ-HRGP precursor (rice) | 148615 | 120 |
| Storage proteins | ||
| Putative bark storage protein (rice) | 140155 | 96 |
| Unknowns | ||
| Unknown protein | BU997476* | 6 |
| Unknown protein | CA031470* | 8 |
| Similar to GP|20160854 P0677H08.8 | BQ463267* | 9 |
| Putative protein | 139132 | 35 |
| Putative protein | 141575 | 41 |
| Putative protein | 148192 | 30 |
| Putative protein | 139730 | 128 |
| Putative protein | 134288 | 40 |
| Unknown protein | 151201 | 53 |
| Hypothetical protein | 151986 | 57 |
| Mucin-like protein (Arabidopsis) | 151069 | 73 |
| No name | 148644 | 101 |
| Hypothetical protein P0461A06.20 | 146050 | 87 |
| No name | BG300785* | 121 |
| No name | 146647 | 90 |
| Unknown protein | 134959 | 92 |
| Unknown protein | 134162 | 98 |
| No name | AU090225* | 113 |
| No name | CA017615* | 80 |
| Osjnba0086b14.2 protein (DUF341 domain) | 138807 | 112 |
| No name | BF260149* | 4 |
| No name | BU999220* | 88 |
| Integral membrane protein (unidentified) | BQ460992* | 122 |
| Similar to Arabidopsis chromosome II BAC T3F17; unknown protein | 146432 | 125 |
| Putative protein | CD663282* | 42 |
| Putative protein | 136440 | 130 |
a
Asterisk denotes NCBI accession number.