Table II.
Protein identification of the R18 eluate analyzed by LC-MS/MS
Mass spectral data were searched against three plant databases. Accession numbers are presented for different databases where the superscript letters denote: A, NCBI-Plants; B, OSGI; and C, ZMGI. Predicted molecular masses correlate with the gel slices from which the proteins were extracted.
| No. | Protein Name | Accession No. | Molecular Mass | Mowse Score (Peptides Matched MS/MS) | Gel ID | Function | Reported | 14-3-3 Motif |
|---|---|---|---|---|---|---|---|---|
| kD | ||||||||
| 1 | Acetyl-CoA carboxylase | gi:2827150A | 254.956 | 110 (2) | 1 | Multifunction | No | KEGTSAP |
| 2 | Gly dehydrogenase | 9629.m04981B | 113.767 | 320 (7) | 1 | Nitrogen metabolism | No | KPSDTFP |
| 3 | Elongation factor 2, putative | 11972.m08296B | 94.015 | 57 (2) | 1 | Translation | No | RLAKSDP |
| 4 | Cytosolic heat shock protein 90 | gi:32765549A | 80.413 | 129 (2) | 1 | Chaperone | Yes (human) | KHFSV/KNDKSV |
| 5 | β-Fructofuranosidase | gi:79326306A | 65.032 | 1,138 (17) | 1 | Glycolysis | Yes | RRSNSW |
| 6 | Neutral invertase-like | gi:18395144A | 63.446 | 581 (8) | 1 | Glycolysis | Yes | KRSASWT |
| 7 | Ulp1 protease family, C-terminal catalytic domain-containing protein | 11981.m05812B | 108.167 | 46 (2) | 2 | Proteolysis | No | RDHFSV/KRSTV |
| 8 | TCP-1/cpn60 chaperonin family | 9634.m00140B | 64.081 | 349 (5) | 2 | Chaperone | No | RRALSYP |
| 9 | Catalase-1 | gi:3929924A | 56.171 | 52 (2) | 2 | Antioxidant | No | RRGSSP |
| 10 | Rubisco large subunit | gi:31087879A | 53.141 | 1,827 (32) | 2 | Calvin cycle | No | – |
| 11 | Elongation factor 1α | TC299030C | 49.137 | 76 (2) | 2 | Translation | No | KMDATTP/KMIPTKP |
| 12 | Transferase family | 9632.m05549B | 47.971 | 60 (2) | 2 | Unknown | No | – |
| 13 | Gln synthetase, catalytic domain | 9632.m05496B | 47.407 | 73 (2) | 2 | Nitrogen metabolism | Yes | KHSSV/KLWSSV/RRPASNM |
| 14 | Phosphoribulokinase | gi:21839A | 45.032 | 388 (6) | 2 | Calvin cycle | No | KLTCSYP |
| 15 | Sedoheptulose-1, 7-bisphosphatase | gi:14265A | 42.057 | 202 (4) | 3 | Calvin cycle | No | KVISV |
| 16 | Fructose-1,6-bisphosphate aldolase precursor | gi:8272480A | 41.919 | 93 (2) | 3 | Glycolysis | No | RTVVSIP/RIPPSVP |
| 17 | GAP dehydrogenase, cytosolic | gi:120668A | 33.233 | 65 (2) | 3 | Glycolysis | Yes | KVIISAP |
| 18 | Short-chain alcohol dehydrogenase | gi:7431022A | 31.644 | 118 (3) | 3 | Signaling | No | – |
| 19 | 14-3-3-like protein | gi:37903393A | 28.880 | 312 (5) | 3 | Signaling | Yes | – |
| 20 | 14-3-3 protein homolog | gi:22607A | 29.262 | 836 (17) | 3 | Signaling | Yes | |
| 21 | Peroxidase, putative | 9635.m04996B | 42.112 | 111 (2) | 4 | Antioxidant | No | RRVLSHP/KIESRP/RVLGSYP |
| 22 | Carbonic anhydrase | gi:558499A | 35.072 | 448 (9) | 4 | Calvin cycle | No | KLIRTTP |
| 23 | Putative acid phosphatase | gi:52346236A | 29.603 | 630 (8) | 4 | Signaling | No | RDWKTVP/RTFSRP |
| 24 | Brain-specific protein | gi:303859A | 29.127 | 114 (2) | 4 | Signaling | Yes | – |
| 25 | Bas1 protein | gi:861010A | 29.090 | 315 (6) | 4 | Antioxidant | No | KSSLSSP/RTLSSP |
| 26 | Thioredoxin peroxidase | gi:3328221A | 28.131 | 325 (6) | 4 | Antioxidant | No | – |
| 27 | ATP-dependent Clp protease proteolytic subunit (endopeptidase Clp) | TC299023C | 27.951 | 78 (2) | 4 | Proteolysis | No | – |
| 28 | Ascorbate peroxidase | gi:15808779A | 27.637 | 252 (5) | 4 | Antioxidant | Yes | KVLLTDP |
| 29 | Rubisco small subunit | gi:4090291A | 19.512 | 383 (7) | 5 | Calvin cycle | No | – |
| 30 | Ser carboxypeptidase S10 family protein, similar to Glc acyltransferase | TC293082C | 51.683 | 51 (2) | 6 | Proteolysis | No | RWLSGE |