Table I.
FNR overexpression in chloroplasts does not affect growth and photosynthetic parameters in transgenic tobacco plants
Tobacco plants from the indicated transgenic lines were germinated in soil and grown for 6 weeks in a growth chamber at two light intensities. Relative FNR amounts were calculated by western-blot analysis. Results are means ± se of four individuals for wild-type and pFNR-1 plants and eight individuals for pFNR-3 plants. Aerial fresh and dry weights were measured in vegetative tissues, including leaves and stem. nd, Not determined.
| Growth Condition
|
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|---|---|---|---|---|---|---|
| 150 μmol quanta m−2 s−1
|
700 μmol quanta m−2 s−1
|
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| Wild Type | pFNR-1 | pFNR-3 | Wild Type | pFNR-1 | pFNR-3 | |
| Relative FNR amount | 1.0 ± 0.2 | 1.1 ± 0.2 | 3.4 ± 0.8 | 1.0 ± 0.1 | 1.0 ± 0.4 | 3.6 ± 0.9 |
| Height (cm) | 57.0 ± 3.0 | 51.7 ± 7.1 | 45.5 ± 3.4 | 43.7 ± 3.9 | 37.7 ± 3.4 | 33.8 ± 1.9 |
| Aerial fresh weight (g) | 82.3 ± 8.0 | 77.5 ± 3.5 | 77.3 ± 5.0 | 52.1 ± 3.4 | 51.9 ± 4.9 | 49.3 ± 2.1 |
| Aerial dry weight (g) | 6.2 ± 0.4 | 6.0 ± 0.7 | 5.0 ± 0.4 | 4.9 ± 0.2 | 4.7 ± 0.5 | 4.3 ± 0.4 |
| Dry weight/fresh weight (%) | 7.5 ± 0.9 | 7.7 ± 2.1 | 6.5 ± 0.9 | 9.4 ± 1.4 | 9.4 ± 1.8 | 8.7 ± 2.4 |
| Chl (μg cm−2) | 37.5 ± 2.9 | 33.4 ± 1.6 | 32.8 ± 3.9 | 18.4 ± 4.4 | 17.7 ± 1.9 | 16.1 ± 4.4 |
| Chl a/b | 2.4 ± 0.1 | 2.4 ± 0.2 | 2.4 ± 0.1 | 2.4 ± 0.2 | 2.3 ± 0.1 | 2.5 ± 0.2 |
| Total carotenoids (μg cm−2) | 6.1 ± 0.4 | 5.5 ± 0.5 | 5.6 ± 0.6 | 4.4 ± 0.5 | 4.2 ± 0.5 | 3.8 ± 0.8 |
| Fv/Fm | 0.79 ± 0.01 | 0.80 ± 0.02 | 0.80 ± 0.01 | 0.78 ± 0.02 | 0.77 ± 0.02 | 0.78 ± 0.02 |
| φPSII at 150 μmol quanta m−2 s−1 | 0.71 ± 0.01 | 0.70 ± 0.01 | 0.70 ± 0.02 | 0.70 ± 0.01 | 0.70 ± 0.01 | 0.7 ± 0.01 |
| φPSII at 1,000 μmol quanta m−2 s−1 | 0.32 ± 0.02 | 0.32 ± 0.06 | 0.31 ± 0.07 | 0.42 ± 0.02 | 0.41 ± 0.04 | 0.41 ± 0.02 |
| NPQ at 150 μmol quanta m−2 s−1 | 0.23 ± 0.03 | 0.23 ± 0.02 | 0.24 ± 0.03 | 0.22 ± 0.03 | 0.20 ± 0.04 | 0.20 ± 0.04 |
| NPQ at 1,000 μmol quanta m−2 s−1 | 1.93 ± 0.14 | 1.83 ± 0.46 | 1.84 ± 0.46 | 1.00 ± 0.19 | 1.22 ± 0.31 | 1.06 ± 0.26 |
| 1 − qP at 150 μmol quanta m−2 s−1 | 0.07 ± 0.01 | 0.10 ± 0.01 | 0.10 ± 0.02 | 0.07 ± 0.01 | 0.05 ± 0.02 | 0.07 ± 0.01 |
| 1 − qP at 1,000 μmol quanta m−2 s−1 | 0.34 ± 0.03 | 0.38 ± 0.04 | 0.41 ± 0.09 | 0.27 ± 0.07 | 0.23 ± 0.05 | 0.30 ± 0.08 |
| ACO2 versus light intensitya | ||||||
| Initial slope | 0.074 ± 0.005 | 0.076 ± 0.011 | 0.071 ± 0.007 | 0.058 ± 0.006 | 0.066 ± 0.004 | 0.068 ± 0.011 |
| Amax (μmol CO2 cm−2 s−1) | 19.43 ± 3.23 | 19.86 ± 2.34 | 19.81 ± 4.22 | 17.23 ± 4.01 | 23.79 ± 4.14 | 19.64 ± 3.11 |
| ACO2 versus Ci at 400 μmol quanta m−2 s−1b | ||||||
| Initial slope | 0.055 ± 0.006 | nd | 0.055 ± 0.010 | 0.101 ± 0.050 | 0.072 ± 0.017 | 0.064 ± 0.016 |
| Amax (μmol CO2 cm−2 s−1) | 18.25 ± 1.91 | nd | 15.89 ± 1.56 | 21.75 ± 1.76 | 19.30 ± 1.49 | 20.84 ± 1.44 |
| ACO2 versus Ci at 2,000 μmol quanta m−2 s−1b | ||||||
| Initial slope | 0.075 ± 0.009 | nd | 0.070 ± 0.006 | 0.059 ± 0.021 | 0.085 ± 0.017 | 0.067 ± 0.030 |
| Amax (μmol CO2 cm−2 s−1) | 31.45 ± 2.31 | nd | 25.51 ± 4.25 | 37.63 ± 5.41 | 35.67 ± 3.36 | 34.90 ± 3.85 |
The net CO2 uptake rate at saturating light (Amax) and the initial slope at low light intensity were derived from light response curves by fitting experimental data to the equation of a nonrectangular hyperbola as described (Hajirezaei et al., 2002).
The net CO2 uptake rate at saturating CO2 internal concentration (Amax) and the initial slope at low Ci were derived from Ci response curves by fitting experimental data to the equation of a nonrectangular hyperbola as described (Hajirezaei et al., 2002).