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. 2007 Jan 25;6:10. doi: 10.1186/1476-4598-6-10

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Copyright © 2007 Menendez et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Methylation analysis of genes upregulated after 5-aza-dC treatment of the OVCAR-3 cell line. Summary of sodium bisulfite sequencing analysis of CGIs associated with the EMP3, CST6, CAV1, MAGEA3 and CXCL6 genes in 5-aza-dC treated and untreated (control) OVCAR-3 cells. Five clones were sequenced for each sample, and each circle displays the percent methylation of all clones for a single CpG dinucleotide (open circle, 100% unmethylated; filled circle, 100% methylated). Average percent methylation across all CpG sites for untreated (control) and 5-aza-dC treated cells is shown next to each row of circles. (Vertical bars, CpG dinucleotides; arrow, position of the transcription start site; horizontal black rectangle, region amplified and sequenced after sodium bisulfite).