Table 3.

Primers used for RT-PCR and Sodium Bisulfite sequencing

Gene	Primer	Sequence	Size	Tm	Ref.
CAV1	RT-PCR_F	5'-CGACCCTAAACACCTCAACGATG-3'	278	55°C	[15]
	RT-PCR-R	5'-GCAGACAGCAAGCGGTAAAACC-3'
	MOD_F	5'-TGTGTATTTTGTAAATATGGTATAATTTG-3'	537	58°C	[15]
	MOD_R	5'-CATTTTCCCTACTCTAAACCAC-3'
SFN	RT-PCR_F	5'-GTGTGTCCCCAGAGCCATGG-3'	161	60°C	[23]
	RT-PCR-R	5'-ACCTTCTCCCGGTACTCACG-3'
CST6	RT-PCR_F	5'-AAGACCAGGGTCACTGGAGA-3'	163	60°C	[12]
	RT-PCR-R	5'-CGGGGACTTATCACATCTGC-3'
	MOD_F	5'-GGTTGGAATGTTGTAGTGGT-3'	430	55°C	[12]
	MOD_R	5'-CCCCAACAACAAATACCAA-3'
MAGEA3	RT-PCR_F	5'-TGGAGGACCAGAGGCCCCC-3'	725	68°C	[16]
	RT-PCR-R	5'-GGACGATTATCAGGAGGCCTGC-3'
	MOD_F	5'-AGATTTGGTTTGAGGGGAGTAGAAGT-3'	405	60°C	[16]
	MOD_R	5'-AACCC(G/A)ACAACAAAAACAACACTAAA-3'
EMP3	RT-PCR-F	5'-TGCTCTCCCTCATTCTCTGCTGTC-3'	274	60°C
	RT-PCR-R	5'-CGCTTCCGTAGGTGGATGTAGATG-3'
	MOD-F	5'-TAGTATATATTGAGAGGAGGAGAG-3'	340	62°C	[17]
	MOD-R	5'-CTTCCCAAACTACTACATTCCCA-3'
CXCL6	RT-PCR-F	5'-CCTGAAGAACGGGAAGC- 3'	135	60°C
	RT-PCR-R	5'-GACTGGGCAATTTTATGATG-3'
	MOD-F	5'-GAGGGATGAATGTAGATAAAGGGAGT-3'	616	60°C
	MOD-R	5'-AACTTCCAAATCCAAACAAACTTACTT-3'
GAPDH	RT-PCR-F	5'-GAAATCCCATCACCATCTTCCAG-3'	312	56°C
	RT-PCR-R	5'-ATGAGTCCTTCCACGATACAAAAG-3'

Size, product size in bp; Tm, annealing temperature