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. 2007 Feb 14;6:16. doi: 10.1186/1476-4598-6-16

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Copyright © 2007 Lin et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Stabilization of p21 in PTEN-knockdown cells iseabrogated by PI3K inhibition with LY-294,002. a. Wild type ACHN cells as well as those stably transfected with PTEN-shRNA or empty vector were incubated with LY-294,002 (10 μM) for the times indicated in the presence of cycloheximide (CHX; 20 μg/ml) for the times indicated, and cell lysate was immunoblotted with antibodies against p21 or β-actin. b. A graphical representation of the data using densitometry of p21 and actin bands is reported as ratio of p21/β-actin and is normalized to the "0 CHX control" for each lane. A representative experiment is shown of three done.