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PTEN knockdown does not alter proteasome-dependent degradation. Cells stably transfected with PTEN-shRNA or empty vector were treated with three different proteasome inhibitors (lactastatin, 20 mM; LLnL, 50 mM; MG132, 1 mM) or cycloheximide (CHX; 20 μg/ml) for 6 h, followed by immunoblotting for p21. A representative experiment is shown of three done.
