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A region required for E2-dependent ubiquitination. (A) Schematic representation of AO7 and AO7 truncations fused to GST at their N termini. The RING and positions of cysteines (C) are indicated. (B) Equimolar amounts of the indicated GST fusions were evaluated for ubiquitination by using 32P-labeled Ub (Upper) or for binding to 35S-labeled UbcH5B (Lower). Upper and Lower are from separate experiments.
