Figure 3.

DTS5 and DTS7 mutations enhance Notch signaling. (A–C) Cuticular preparations of dorsal thoraces from H^E31 heterozygous pharate adults. Removing one dose of Hairless strongly enhances the effect of the DTS5 and DTS7 mutations in pupae heat-pulsed at 29°C during a 0- to 24-hr-APF time period: compare H^E31/DTS5 (B) and H^E31/DTS7 (C) with control H^E31/+ pharate adults (A). No interaction is seen at the permissive temperature (not shown). (D–F) Cuticular preparations of dorsal thoraces from P[w⁺, hs-Nintra]-I4A pharate adults. Wild-type (D), DTS5/+ and DTS7/+ 0-hr-APF pupae, carrying one copy of a hs-Nintra transgene, were placed at 29°C for 24 hr and then heat-shocked at 37°C for 20 min. Expression of Nintra at 24 hr APF leads to a relatively mild double-socket phenotype. The DTS5 and DTS7 mutations strongly enhanced the double-socket phenotype produced by Nintra (compare E and F with D).