Table 2.
Plasmids used in this study.
| Name | Relevant fragment | Comment | Origin/marker | Reference |
|---|---|---|---|---|
| pJV861-9 | PLtetO-lacZ::gfp | lacZ transcriptional GFP fusion plasmid | pSC101*/CmR | Urban and Vogel (2006) |
| pJV783-1 | PmicA-gfp | micA transcriptional GFP fusion plasmid | pSC101*/CmR | This study |
| pJV784-25 | PrybB-gfp | rybB transcriptional GFP fusion plasmid | pSC101*/CmR | This study |
| pJV990 | luc | pZE12-luc derivate, ColE1 origin exchanged pSC101* of pZS*24-MCS-1 | pSC101*/AmpR | This study and Lutz and Bujard (1997) |
| pAS0046 | gfp | Transcriptional fusion vector, based on pJV859-8 | pSC101*/CmR | Sittka et al. (2006) |
| pJV790 | PLtetO-lacZ::gfp | lacZ transcriptional GFP fusion plasmid | pSC101*/AmpR | This study |
| pJV791 | PmicA-gfp | micA transcriptional GFP fusion plasmid | pSC101*/AmpR | This study |
| pJV792 | PrybB-gfp | rybB transcriptional GFP fusion plasmid | pSC101*/AmpR | This study |
| pBAD/Myc-His A | pBAD expression plasmid | pBR322/AmpR | Invitrogen | |
| pKP8-35 (pBAD) | pBAD control plasmid, expresses ∼50 nt nonsense RNA derived from rrnB terminator | pBR322/AmpR | This study | |
| pKP17-1 (pBAD-RybB) | rybB | RybB expression plasmid, rybB is controlled by the plasmid-borne PBAD promoter | pBR322/AmpR | This study |
| pBAD33 | pBAD expression plasmid | pBR322/CmR | Guzman et al. (1995) | |
| pAC-rpoEST4 (pBAD-RpoE) | rpoE | RpoE expression plasmid, rpoE is controlled by the plasmid-borne PBAD promoter | pBR322/CmR | Miticka et al. (2003) |
| pKD3 | Template for mutant construction; carries chloramphenicol-resistance cassette | oriRγ/AmpR | Datsenko and Wanner (2000) | |
| pKD4 | Template for mutant construction; carries kanamycin-resistance cassette | oriRγ/AmpR | Datsenko and Wanner (2000) | |
| pKD46 | ParaB-γ-β-exo | Temperature-sensitive lambda-red recombinase expression plasmid | oriR101/AmpR | Datsenko and Wanner (2000) |
| pCP20 | Temperature-sensitive FLP recombinase expression plasmid | oriR101/AmpR, CmR | Datsenko and Wanner (2000) |