Table II.
Peptides recognized by vaccinia-specific CD8 T cellsa
| ORF | Epitope | Sequence | HLA Allele | Orthopox Conservationb | Molluscum Contagiosum Conservationc |
|---|---|---|---|---|---|
| A3L | 90–98 | DEVASTHDW | B*4403 | Primate OP all (+) | DEVASTQDW 8/9 |
| A3L | 264–272 | YEFRKVKSY | B*4403 | Primate OP all (+) | YELKKVRPD 4/9 |
| A23R | 287–295 | HDVYGVSNF | B*4403 | Primate OP all (+) | AHMYYGVHNF 5/9 |
| A24R | 278–286 | ITDFNIDTY | A*0101 | Primate OP all (+) | EDDFDVAEY 3/9 |
| A48R | 58–66 | TYNDHIVNL | A*2301 | Primate OP all (+) | No homolog |
| A50R | 395–403 | WLKIKRDYL | B*0801 | Primate OP all (+) | No homolog |
| C12L | 326–334 | VYINHPFMY | A*2902 | Fragmented in MVA (31) | No homolog |
| D1R | 126–134 | EERHIFLDY | B*4403 | Primate OP all (+) | EEQYVFLDF 5/9 |
| D5R | 298–306 | LENGAIRIY | B*4403 | Primate OP all (+) | LGNGALRIF 6/9 |
| D5R | 691–699 | EEIPDFAFY | B*4403 | Primate OP all (+) | DLIPDFCFQ 5/9 |
| D5R | 349–357 | VWINNSWKF | A*2301 | Primate OP all (+) | VWLRNCWRF 5/9 |
| E3L | 86–94 | DDVSREKSM | B*4403 | Primate OP all (+) | No homolog |
| F3 | 41–49 | EEQELLLLY | B*4403 | Primate OP all (+) | No homolog |
| I3L | 173–181 | IEGELESLS | B*4403 | Primate OP all (+) | MLRELETLA 4/9 |
| IL-18bpd | 21–29 | DEIKCPNLN | B*4403 | Copen (−), MVA, variola, monkeypox (each divergent) | No homolog |
| M2L | 38–46 | AELTIGVNY | B*4403 | Deleted in NYVAC | No homolog |
CD8 CTL clones were tested in 51Cr-release assays. Bulk CTL were tested for IFN-γ release and/or IFN-γ accumulation by ICC and only peptides with two or more positive tests are listed.
Data from Ref. 21 and J. Tartaglia (unpublished observation). Primate orthopox (OP) analyzed were the primate orthopoxviruses vaccinia Copenhagen, vaccinia Western Reserve, MVA Acambis 3000, monkeypox (MP) Zaire, monkeypox Congo, variola major India, and NYVAC (24). (+), Peptide epitope predicted to be expressed; (−), peptide epitope either altered or deleted and therefore not predicted to be expressed.
Data from Ref. 21. The sequence of the homologous region of the homologous protein, if any, is shown, followed by the number of identical amino acids at orthologous positions and the total number of amino acids.