Table III.
Regions of vaccinia ORFs that contain putative epitopes stimulating human HLA class I-restricted CD8+ T cellsa
| ORFb | HLA cDNA | Predicted Amino Acidsb |
|---|---|---|
| A3L | B*4403 | 487–567 |
| A3L | B*4403 | 393–474 |
| A24R | A*0101 | 747–897 |
| A57R | A*2301 | 1–62 |
| F12L | A*2301 | 147–280 |
| F12L | A*0101 | 392–486 |
| IL-18bpc | A*0101 | 59–126 |
Each fragment was repeatedly positive after cotransfection with indicated HLA cDNA. Recognition of an internal peptide has not yet been demonstrated.
Synthesis of data from sequence of biologically active plasmid(s). If more than one nonidentical plasmid was recovered, the shortest overlapping region is reported. If one or more plasmids was out-of-frame with eGFP, internal AUG codons are considered the 5′ limit of regions possibly encoding an epitope or epitopes.
The SOR was homologous to aa 59–126 of vaccinia WR VACWR013, also called C12L in this strain. The predicted vaccinia strain NYCBH 59–162 from our sequencing is divergent at the predicted amino acid level from homologous region of vaccinia WR.