Figure 5. 53BP1 Localization to Sites of DNA DSBs Is Mediated by the Interaction between 53BP1 Tandem Tudor Domains and Dimethylated Lys20 of Histone H4.
53BP1 relocalization (53BP1) in irradiated 53BP1 null MEFs transiently transfected with 53BP1 wild-type (WT) or 53BP1 mutated in the H4-K20me2-binding cage. Sites of DNA DSBs were marked by costaining using anti-phosphorylated-H2AX (Ser139) (P-H2AX) antibody.