NV1066 selectively infects malignant mesothelioma and spares normal tissue. Tissue specimens from thoracic cavity were selected by their GFP expression. Serial sectioning was performed and specimens were stained with polyclonal HSV-1 antibody. Representative section shown here includes mesothelioma nodule (A, HSV immunohistochemistry and B, H&E), HSV immunohistochemistry of normal tissue from brain (C), lung (D), intestine (E), liver (F), spleen (G) and bone (H). Viral infection was localized to mesothelioma nodules. No GFP expression or viral staining was evident in normal tissue surrounding mesothelioma nodules as shown in figures I and J. GFP = Green fluorescent protein