Figure 1.

Sgo2 does not protect centromeric cohesion in fission yeast mitosis. (A) nda3-KM311 rad21⁺-GFP cells carrying sgo2Δ or not were arrested at prometaphase and fixed for ChIP analysis. Rad21-GFP levels throughout the indicated chromosome sites were measured by ChIP analysis with anti-GFP. The quality of the prometaphase arrest was determined by staining for histone H3-Ser-10 phosphorylation (H3S10ph). Note that sgo2Δ cells as well as sgo2⁺ cells largely arrested at prometaphase by nda3-KM311 inactivation, whereas checkpoint-defective mad2Δ cells did not (also see Supplementary Fig. 1). Error bars represent the SD of duplicate PCRs. (B) The indicated strains cultured at 25°C were shifted to 36°C for 4 h (metaphase arrest), fixed, and stained for tubulin and cen2-GFP. The distance between cen2-GFP dots was measured. Error bars represent the SD of duplicate samples (each n > 50).