Figure 3.

Metabolic rate and metabolic output of wild-type, long-lived daf-2 worms and of daf-2 worms genetically suppressed by a mutation in the daf-16 gene. daf-2;daf-16 worms are identical to daf-2 worms, except that they have an additional mutation in the daf-16 gene. This gene is required for worms to form dauer larvae. (a) Metabolic rate of wild-type, daf-2;daf-16, and daf-2 worms. Bars represent the means (± SEM) of four measurement values per temperature, with 50 worms used per measurement. (b) Metabolic output of wild-type, daf-2;daf-16, and daf-2 worms. Metabolic output was calculated as described in Fig. 2c. Each bar represents the mean (± SEM) of four integration values with each integration value calculated from metabolic rates measured at five time points.