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. 1999 Sep 28;96(20):11404–11409. doi: 10.1073/pnas.96.20.11404

Figure 4.

Figure 4

In situ assay demonstrates detergent-resistant insolubility of extended polyQ aggregates and coaggregates. (A) Low-power view of 25Q/EGFP cotransfected with 104Q (not stained), demonstrating aggregation of normal length polyQ by extended polyQ. (B) 25Q/EGFP transfected alone and treated with 2.5% SDS/2.5% Triton X-100 for 2 min. Normal length polyQ alone does not aggregate and EGFP fluorescence is not preserved. (C) 25Q/EGFP cotransfected with 104Q (not stained), treated with 2.5% SDS/2.5% Triton X-100. Aggregates remained fluorescent for up to 24 hr of detergent treatment. (D) Lower-power view of stable inducible live culture expressing 104Q/EGFP (green) and forming aggregates. (E) Same field as A after 2-min treatment with 2.5% SDS/2.5% Triton X-100. Aggregates were similar in size, number, and fluorescent intensity even after 24 hr of treatment at 37°C. (F) Low-power view of stable inducible live culture expressing 25Q/EGFP. (G) Same field as C after 2-min treatment with detergent as above. No remaining fluorescence is detectable.