Figure 3.
Relative activity of wild-type and hyperactive Himar1 transposases as measured in the mating-out assay. The frequency of transposition is expressed relative to that of wild-type Himar1 transposase, which has been normalized to a value of 1.0. A typical transposition frequency for wild-type transposase under the conditions described here is ≈4 × 10−6. Relative frequencies are calculated by dividing each of the absolute frequencies by the average absolute transposition frequency of wild-type Himar1 transposase. The errors are SEM. Relative errors are computed by dividing the absolute errors by the mean absolute frequency for the wild-type transposase.