Table 1.
Bacterial strains and plasmids used in this study
| Strains, plasmids | Description | Reference or Supplier |
|---|---|---|
| Strains | ||
| β2155 | thrB1004 pro thi strA hsdS lacZΔM15 | 28 |
| (F′ lacZΔM15 lacI9 traD36 proA+ proB+) | ||
| ΔdapA∷erm (=EmR) pir∷RP4 [∷kan (KmR) from SM10] | ||
| HB101 | supE44 hsdS20 (rB−mB−) recA13 ara-14 | 50 |
| proA2 acY1 galK2 rpsL20 xyl-5 mtl-1 | ||
| HBfLac | HB101 F∷miniHimar1LacTet from pMMLacTet | This study |
| DH5α | Δ(lac)U169 endA1 gyrA96 hsdR17 recA1 | 51 |
| relA1 supE44 thi-1 φ80lacZΔM15 | ||
| DL1 | DH5α F∷miniHimar1LacTet from pMMLacTet | This study |
| RZ212/pOX38-Gen | D(lac-pro), ara, str, recA56, srl, thi, | 52 |
| RZ212MK | RZ212/ pACMarKan (mating-out strain) | This study |
| RZ221 | polA, Δ(lac-pro), ara, str, nal | 53 |
| BL21(DE3) | F−, omp T, rB− mB− l DE3 | 54 |
| Plasmids | ||
| pMMOrf | Like pMMar but with ORF through 3′ ITR | This study |
| pMMLacTet | pMMOrf containing lacZYA and Tetr gene | This study |
| pBCMAR | Himar1 coding sequence under Plac control | This study |
| pMarNco | Himar1 coding sequence with NcoI at start site | This study |
| pRZ1495 | Tn5 papillation factor | 26 |
| p27fH-5′ | pK19 containing left (5′) ITR of Himar1 | 10 |
| pACMarKan | pACYC184 carrying KanrHimar1 | This study |
| pMarNde18 | Himar1 lacking ITR sequences | 10 |
| pMinimariner | Himar1 of only the first and last 100 bp | 13 |
| pBAD24 | Expression plasmid with araBAD promoter | 30 |
| pBADMar1 | Himar1 tpase under araBAD promoter | This study |
| pBADH267R | As pBADMar1 but with H267R mutation | This study |
| pBADC9 | As pBADMar1 but with Q131R and E137K mutations in Himar1 tpase | This study |
| pET29A7 | pET29b+ carrying H267R mutation in Himar1 tpase | This study |
| pET29C9 | pET29b+ carrying Q131R and E137K mutations in Himar1 tpase | This study |
| pCDNAII | Target plasmid for in vitro reactions | Invitrogen |