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. 2007 Mar;18(3):995–1008. doi: 10.1091/mbc.E06-08-0685

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© 2007 by The American Society for Cell Biology

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Figure 4. — Analysis of autofluorescent, acidified, and terminal endocytic compartments in intestinal cells of L4/adult stage pgp-2(−) animals. The autofluorescent contents of wild-type gut granules are visible in the rhodamine channel (B). After staining wild type with dyes that label acidified (D) or terminal endocytic compartments (F), gut granules displayed brighter fluorescence. The intestinal cells of pgp-2(kx48) animals did not contain autofluorescent material visible in the rhodamine channel (H), and they did not stain with the acidic marker Lysotracker Red (J). The endocytic marker TRITC-BSA accumulated in pgp-2(kx48) intestinal cells at much lower levels than wild type (compare F and L). Fluorescence images of wild-type and pgp-2(kx48) were captured using the same exposure time. The intestinal lumen is marked with a black arrow.