Table 1.
Birefringent and autofluorescent gut granules
| Genotype | % of embryos with the specified number of birefringent granules in intestinal cells |
% of L4/young adults with the specified number of autofluorescent organelles in intestinal cells |
||||
|---|---|---|---|---|---|---|
| None | 1–100 | >100a | 0–10 | 11–100 | >100a | |
| Wild type | 0 | 0 | 100 (>200) | 0 | 0 | 100 (>200) |
| ABCB transporters | ||||||
| pgp-1(pk17) | 0 | 0 | 100 (40) | 0 | 0 | 100 (20) |
| pgp-2(kx48) | 0 | 96 | 4 (83) | 0 | 100 | 0 (25) |
| pgp-2(kx55) | 0 | 92 | 8 (75) | 0 | 100 | 0 (25) |
| pgp-2(gk114) | 0 | 94 | 6 (68) | 0 | 100 | 0 (20) |
| pgp-2(RNAi)c | 0 | 100 | 0 (62) | 0 | 100 | 0 (23) |
| pgp-3(pk18) | 0 | 0 | 100 (34) | 0 | 0 | 100 (20) |
| pgp-4(gk17) | 5 | 0 | 95 (42) | 0 | 0 | 100 (20) |
| pgp-5(ok663) | 0 | 0 | 100 (45) | 0 | 0 | 100 (20) |
| pgp-6(ok994) | 0 | 0 | 100 (40) | 0 | 0 | 100 (20) |
| pgp-7(ok528)d | 0 | 0 | 100 (200) | 0 | 0 | 100 (24) |
| pgp-8(RNAi)c | 0 | 0 | 100 (40) | 0 | 0 | 100 (24) |
| pgp-9(RNAi)c | 0 | 0 | 100 (50) | 0 | 0 | 100 (25) |
| pgp-10(ok991) | 0 | 2 | 98 (47) | 0 | 0 | 100 (25) |
| pgp-11(RNAi)c | 0 | 0 | 100 (45) | 0 | 0 | 100 (20) |
| pgp-12(gk19) | 0 | 0 | 100 (40) | 0 | 0 | 100 (25) |
| pgp-13(ok747) | 0 | 0 | 100 (50) | 0 | 0 | 100 (20) |
| pgp-14(RNAi)c | 0 | 0 | 100 (40) | 0 | 0 | 100 (25) |
| pgp-15(ok987) | 0 | 0 | 100 (50) | 0 | 0 | 100 (20) |
| haf-1(ok705) | 0 | 0 | 100 (50) | 0 | 0 | 100 (25) |
| haf-2(gk13) | 0 | 0 | 100 (50) | 0 | 0 | 100 (20) |
| haf-3(ok1086) | 0 | 0 | 100 (57) | 0 | 0 | 100 (25) |
| haf-4(gk240) | 53 | 31 | 16 (55) | 0 | 0 | 100 (25) |
| haf-5/hmt-1(gk155) | 0 | 0 | 100 (55) | 0 | 0 | 100 (25) |
| haf-5/hmt-1(gk161) | 5 | 0 | 95 (56) | 0 | 0 | 100 (24) |
| haf-7(gk46) | 0 | 0 | 100 (50) | 0 | 0 | 100 (25) |
| haf-8(gk12) | 0 | 0 | 100 (50) | 0 | 0 | 100 (20) |
| haf-9(gk23) | 2 | 0 | 98 (47) | 0 | 0 | 100 (20) |
| Double mutant analysise | ||||||
| apt-6(ok429) | 5 | 82 | 13 (98) | 0 | 100 | 0 (25) |
| apt-6(ok429) pgp-2(kx48) | 99 | 1 | 0 (86) | 100 | 0 | 0 (25) |
| apt-7(tm920) | 10 | 88 | 2 (98) | 0 | 100 | 0 (25) |
| apt-7(tm920); pgp-2(kx48) | 93 | 7 | 0 (92) | 100 | 0 | 0 (25) |
| apt-7(tm920); pgp-2(kx55) | 100 | 0 | 0 (72) | 100 | 0 | 0 (28) |
| glo-1(zu437) | 100 | 0 | 0 (84) | 100 | 0 | 0 (20) |
| glo-1(zu437); pgp-2(kx48) | 100 | 0 | 0 (65) | 100 | 0 | 0 (27) |
| glo-4(ok623) | 100 | 0 | 0 (64) | 100 | 0 | 0 (25) |
| glo-4(ok623); pgp-2(kx48) | 100 | 0 | 0 (71) | 100 | 0 | 0 (27) |
All strains were grown at 22°C. Two-fold and later stage embryos were analyzed using polarization microscopy and scored for the number of birefringent gut granules within the intestine. L4/young adults were analyzed using fluorescence microscopy with a standard fluorescein isothiocyanate (FITC) filter and were scored for the number of autofluorescent gut granules present within the intestine.
a Values in parentheses are the number of embryos scored.
b Values in parentheses are the number of animals scored.
c RNAi was carried out in an rrf-3(pk1426) RNAi-sensitive background (Simmer et al., 2002).
d Embryos scored were the progeny of pgp-7(ok528)/szT1 balanced strain, pgp-7(ok528) homozygous adults with protruding vulvas that produced dead embryos were scored.
e A dpy-5(e61) marker that did not alter the Glo phenotype was present in each strain.