Table 2.
Rescue of pgp-2(kx48)
| Genotype | Autofluorescent organelles | Acridine orange–stained organelles | Nile Red–stained organelles |
|---|---|---|---|
| Wild type | +++ (18) | +++ (20) | +++ (17) |
| pgp-2(kx48) | − (22) | − (19) | − (27) |
| pgp-2(kx48) + vha-6::pgp-2::gfp | +++ (23) | +++ (46) | +++ (16) |
| pgp-2(kx48) + unc-119::pgp-2::gfp | − (22) | − (30) | − (32) |
| pgp-2(kx48) + vha-6::pgp-2(K440R)::gfp | + (37)a | − (19) | + (17)b |
| pgp-2(kx48) + vha-6::pgp-2(K1069R)::gfp | + (20)a | − (15) | + (22)b |
| pgp-2(kx48) + vha-6::pgp-2(K440R; K1069R)::gfp | + (20)a | − (19) | + (19)b |
All strains were grown at 22°C. L4/young adults were analyzed using fluorescence microscopy for autofluorescent, acridine orange, and Nile Red signals in intestinal cells using a standard rhodamine filter. Animals were scored for the number of fluorescent organelles present within the intestine. +++, ++, +, and − correspond to >500, 51–500, 1–50, and 0 organelles, respectively. Values in parentheses are the number of animals scored.
a pgp-2 point mutants contained weakly autofluorescent compartments compared with wild type.
b pgp-2 point mutants contained weakly stained Nile Red–stained compartments compared with wild type.