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. 2007 Mar;18(3):1073–1082. doi: 10.1091/mbc.E06-05-0406

Figure 1.

Figure 1.

aa931-1039 of mTOR is sufficient to target EGFP to the Golgi apparatus. (A) Schematic presentation of mTOR and subcellular localization of two mTOR N-terminal fragments in HeLa cells. (B) aa931-1039-EGFP is predominantly localized to the Golgi in HeLa cells. EGFP or aa931-1039-EGFP is transiently expressed in HeLa cells (green). The Golgi is visualized by IF staining with a Golgin-97–specific antibody (red). The nuclei are stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). (C) aa931-1039-EGFP is localized to the Golgi in several common mammalian cell lines. aa931-1039-EGFP is transiently expressed in HEK293T, RH30, and MRC-5 cells (green). The Golgi is visualized by IF with a Golgin-97–specific antibody (red). The nuclei are stained with DAPI (blue).