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αA-crystallin overexpression has no effect on the biogenesis of wild-type CFTR. HEK293 cells were transfected with 1.5 μg of pcDNA3.1-CFTR and 0.5 μg of pcDNA3.1 or pcDNA3.1-αA-crystallin per 60-mm dish. Rates of CFTR maturation (C-band) and CFTR B-band degradation as well as αA-crystallin expression levels were determined by pulse-chase immunoprecipitation in cells transfected with CFTR and containing a vector control or the αA-crystallin expressing vector.
