Figure 10.

DACH1 repression of c-Jun activity is rescued in trans by c-Jun requiring the N terminus. (A) HEK293T cells were transiently transfected with a multimeric E2 binding site linked to a luciferase reporter gene (E2)₆-LUC and mammalian expression vectors encoding c-Jun-E2, DACH1 or c-Jun. (B) Schematic representation of mammalian c-Jun expression vectors. (C) Western blot analysis of HEK293T cells transiently transfected with c-Jun wild-type or mutant expression vectors with DACH1. HA epitope of DACH1 expression vector was used to immunoprecipitate DACH1. Coprecipitation of c-Jun is shown using a c-Jun–specific antibody. (D) Rescue of DACH1 repression by mammalian c-Jun mutant expression vectors. Rescue is shown as a percentage of wild-type c-Jun for each mutant. Data are mean ± SEM of n = 6.