Figure 7.

DACH1 inhibition of c-fos promoter activity requires the SRE, but not TCF site. (A) Schematic representation of c-fos promoter luciferase reporter vectors, indicating the TCF and SRE sites. (B) 3T3 cells were transiently transfected with a c-fos wild-type or mutant promoter reporter constructs and stimulated by serum for 7 h as shown. 3T3 cells were transiently transfected with c-fos promoter point mutant PM18 (C) or PM12 (D) luciferase reporter genes, and expression vectors encoding DACH1 wild-type or mutant. MCF-7 cells were transiently transfected with either wild-type or mutant c-fos promoter luciferase reporters and treated with TPA for 3 or 7 h (E) or cotransfected with a mammalian expression vector for DACH1 (F). All changes showing mean ± SEM of n = 6 separate transfections.