Figure 9.

DACH1 and c-Jun interact in mammalian two-hybrid system. (A) Schematic representation of c-Jun protein linked to a Gal4 binding domain or DACH1, linked to the VP16 transactivation domain. HEK293T cells were transfected with a multimeric luciferase reporter gene UAS₅ E1B TATA-LUC and mammalian expression vectors encoding the Gal4 DNA-binding domain linked to c-Jun with VP16-DACH1 (B) or DACH1 (C). HEK293T cells were transiently transfected with a luciferase reporter UAS₅ E1BTATA-LUC and mammalian expression vectors for c-Jun, Ras V12, or DACH1 as shown (D–E). Data are mean ± SEM of n = 7 separate transfections normalized to the β-galactosidase for transfection efficiency.