Table 1.

FISH replication-timing patterns of different probes in LAZ cells

Probe	% D.D.	% S.D.	% S.S.	No. scored	Comments
CL15	60.5	10.5	29.0	104	Early control
cJ21	22.0	13.5	64.5	124	Late control
N85E7	42.0	11.5	46.5	105
N85A3	18.0	13.5	68.5	105

Nuclei from LAZ cells were prepared and hybridized in situ to various cosmid probes: CL15, muscle glycogen phosphorylase (PYGM) gene; cJ21, cystic fibrosis transmembrane region gene; N85E7 and N85A3, probes from the 22q telomeric region (see Fig. 1a). Percentages of nuclei exhibiting each of the three patterns of hybridization (D.D., S.D., S.S.) are indicated together with the total number of nuclei scored for each probe.